Among the driving factors for the high production cost of cellulosic butanol lies the pretreatment and product separation sections, which often demand high amounts of energy, chemicals, and water. In this study, techno-economic analysis of several pretreatments and product separation technologies were conducted and compared. Among the pretreatment technologies evaluated, low-moisture anhydrous ammonia (LMAA) pretreatment has shown notable potential with a pretreatment cost of $0.16/L butanol. Other pretreatment technologies evaluated were autohydrolysis, soaking in aqueous ammonia (SAA), and soaking in sodium hydroxide solution (NaOH) with pretreatment costs of $1.98/L, $3.77/L, and $0.61/L, respectively. Evaluation of different product separation technologies for acetone-butanol-ethanol (ABE) fermentation process have shown that in situ stripping has the lowest separation cost, which was $0.21/L. Other product separation technologies tested were dual extraction, adsorption, and membrane pervaporation, with the separation costs of $0.38/L, $2.25/L, and $0.45/L, respectively. The evaluations have shown that production of cellulosic butanol using combined LMAA pretreatment and in situ stripping or with dual extraction recorded among the lowest butanol production cost. However, dual extraction model has a total solvent productivity of approximately 6% higher than those of in situ stripping model.