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Complete genome sequence of the lignin-degrading bacterium Klebsiella sp. strain BRL6-2
Standards in Genomic Science
  • Hannah L. Woo, University of Tennessee, Knoxville
  • Nicholas R. Ballor, Joint BioEnergy Institute
  • Terry C. Hazen, University of Tennessee, Knoxville
  • Julian L. Fortney, University of Tennessee, Knoxville
  • Blake Simmons, Joint BioEnergy Institute
  • Karen Walston Davenport, Los Alamos National Laboratory
  • Lynne Goodwin, Los Alamos National Laboratory
  • Natalia Ivanova, Department of Energy Joint Genome Institute
  • Nikos C. Kyrpides, Department of Energy Joint Genome Institute
  • Konstantinos Mavromatis, Department of Energy Joint Genome Institute
  • Tanja Woyke, Department of Energy Joint Genome Institute
  • Janet Jansson, Pacific Northwest National Laboratory
  • Jeff Kimbrell, Joint BioEnergy Institute
  • Kristen DeAngelis, University of Massachusetts Amherst
Publication Date

In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated Klebsiella sp. strain BRL6-2 on minimal media with alkali lignin as the sole carbon source. This organism was isolated anaerobically from tropical forest soils collected from the Bisley watershed at the Ridge site in the El Yunque National Forest in Puerto Rico, USA, part of the Luquillo Long-Term Ecological Research Station. At this site, the soils experience strong fluctuations in redox potential and are characterized by cycles of iron oxidation and reduction. Genome sequencing was targeted because of its ability to grow on lignin anaerobically and lignocellulolytic activity via in vitro enzyme assays. The genome of Klebsiella sp. strain BRL6-2 is 5.80 Mbp with no detected plasmids, and includes a relatively small arsenal of genes encoding lignocellulolytic carbohydrate active enzymes. The genome revealed four putative peroxidases including glutathione and DyP-type peroxidases, and a complete protocatechuate pathway encoded in a single gene cluster. Physiological studies revealed Klebsiella sp. strain BRL6-2 to be relatively stress tolerant to high ionic strength conditions. It grows in increasing concentrations of ionic liquid (1-ethyl-3-methyl-imidazolium acetate) up to 73.44 mM and NaCl up to 1.5 M.

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Citation Information
Hannah L. Woo, Nicholas R. Ballor, Terry C. Hazen, Julian L. Fortney, et al.. "Complete genome sequence of the lignin-degrading bacterium Klebsiella sp. strain BRL6-2" Standards in Genomic Science Vol. 9 (2014)
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