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Complete genome sequence of “Enterobacter lignolyticus” SCF1
Standards in Genomic Sciences
  • Kristen DeAngelis, University of Massachusetts Amherst
  • Patrick D'Haeseleer, Lawrence Berkeley National Laboratory
  • Dylan Chivian, Joint BioEnergy Institute
  • Julian L. Fortney, Lawrence Berkeley National Laboratory
  • Jane Khudyakov, Joint BioEnergy Institute
  • Blake Simmons, Joint BioEnergy Institute
  • Hannah Woo, Lawrence Berkeley National Laboratory
  • Adam P. Arkin, Joint BioEnergy Institute
  • Karen Walston Davenport, Los Alamos National Laboratory
  • Lynne Goodwin, Los Alamos National Laboratory
  • Amy Chen, Department of Energy Joint Genome Institute
  • Natalia Ivanova, Department of Energy Joint Genome Institute
  • Nikos C. Kyrpides, Department of Energy Joint Genome Institute
  • Konstantinos Mavromatis, Department of Energy Joint Genome Institute
  • Tanja Woyke, Department of Energy Joint Genome Institute
  • Terry C. Hazen, Joint BioEnergy Institute
Publication Date
2011
Abstract

In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated Enterobacter lignolyticusSCF1 on minimal media with alkali lignin as the sole source of carbon. This organism was isolated anaerobically from tropical forest soils collected from the Short Cloud Forest site in the El Yunque National Forest in Puerto Rico, USA, part of the Luquillo Long-Term Ecological Research Station. At this site, the soils experience strong fluctuations in redox potential and are net methane producers. Because of its ability to grow on lignin anaerobically, we sequenced the genome. The genome of E. lignolyticus SCF1 is 4.81 Mbp with no detected plasmids, and includes a relatively small arsenal of lignocellulolytic carbohydrate active enzymes. Lignin degradation was observed in culture, and the genome revealed two putative laccases, a putative peroxidase, and a complete 4-hydroxyphenylacetate degradation pathway encoded in a single gene cluster.

Disciplines
DOI
10.4056/sigs.2104875
Pages
69-85
Creative Commons License
Creative Commons Attribution-Share Alike 4.0
Citation Information
Kristen DeAngelis, Patrick D'Haeseleer, Dylan Chivian, Julian L. Fortney, et al.. "Complete genome sequence of “Enterobacter lignolyticus” SCF1" Standards in Genomic Sciences Vol. 5 (2011)
Available at: http://works.bepress.com/kristen_deangelis/18/