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Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov.
Standards in Genomic Science
  • Andrew F. Billings, University of Massachusetts Amherst
  • Julian L. Fortney, University of Tennessee, Knoxville
  • Terry C. Hazen, University of Tennessee, Knoxville
  • Blake Simmons, Joint BioEnergy Institute
  • Karen W. Davenport, Los Alamos National Laboratory
  • Lynne Goodwin, Los Alamos National Laboratory
  • Natalia Ivanova, Department of Energy Joint Genome Institute
  • Nikos C. Kyrpides, Department of Energy Joint Genome Institute
  • Konstantinos Mavromatis, Department of Energy Joint Genome Institute
  • Tanya Woyke, Department of Energy Joint Genome Institute
  • Kristen DeAngelis, University of Massachusetts Amherst
Publication Date
2015
Abstract

Tolumonas lignolytica BRL6-1T sp. nov. is the type strain of T. lignolytica sp. nov., a proposed novel species of the Tolumonas genus. This strain was isolated from tropical rainforest soils based on its ability to utilize lignin as a sole carbon source. Cells of Tolumonas lignolytica BRL6-1T are mesophilic, non-spore forming, Gram-negative rods that are oxidase and catalase negative. The genome for this isolate was sequenced and returned in seven unique contigs totaling 3.6Mbp, enabling the characterization of several putative pathways for lignin breakdown. Particularly, we found an extracellular peroxidase involved in lignin depolymerization, as well as several enzymes involved in β-aryl ether bond cleavage, which is the most abundant linkage between lignin monomers. We also found genes for enzymes involved in ferulic acid metabolism, which is a common product of lignin breakdown. By characterizing pathways and enzymes employed in the bacterial breakdown of lignin in anaerobic environments, this work should assist in the efficient engineering of biofuel production from lignocellulosic material.

Disciplines
DOI
10.1186/s40793-015-0100-3
Creative Commons License
Creative Commons Attribution 4.0
License
UMass Amherst Open Access Policy
Citation Information
Andrew F. Billings, Julian L. Fortney, Terry C. Hazen, Blake Simmons, et al.. "Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov." Standards in Genomic Science Vol. 10 (2015)
Available at: http://works.bepress.com/kristen_deangelis/16/