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Article
Characterization of the Differentiation and Leptin Secretion Profile of Adult Stem Cells on Patterned Polylactide Films
Journal of Biomaterials Science, Polymer Edition
  • Aditya Chaubey, Clemson University
  • Kevin J Ross, University of North Carolina at Chapel Hill
  • Malcolm R Leadbetter, University of North Carolina at Chapel Hill
  • Cheryl T Gomillion, Clemson University
  • Karen J.L. Burg, Clemson University
Publication Date
4-2-2012
Abstract
Several issues need to be better understood before breast tissue engineering becomes a clinically viable option. One of the most important aspects is the interaction between cells and the microtopography of the implant surface. The aim of this study was to evaluate the efficacy of D1 cells, multipotent mouse bone marrow stromal precursors, in differentiating to adipocytes and to characterize their metabolic activity (lactic acid released and glucose consumed), leptin secretion and lipid production when cultured on patterned poly(L-lactide) (PLLA) films. It was determined that, by appropriate stimulation, the D1 cells displayed morphological characteristics of adipocytes and produced lipid. The results showed that a patterned surface did affect the rate of lipid production. Polynomial models were proposed to predict the amount of leptin secreted by the cells over a period of time.
Publisher statement
This is an electronic version of an article published in Journal of Biomaterials Science, Polymer Edition.
Citation Information
Aditya Chaubey, Kevin J Ross, Malcolm R Leadbetter, Cheryl T Gomillion, et al.. "Characterization of the Differentiation and Leptin Secretion Profile of Adult Stem Cells on Patterned Polylactide Films" Journal of Biomaterials Science, Polymer Edition Vol. 20 Iss. 7-8 (2012) p. 1163 - 1177
Available at: http://works.bepress.com/kjross/4/