In the model nematode system C. elegans, one area of sexual differentiation among cells are the neuronal cells of hermaphrodites and males, where the males have more neurons used in various aspects of mating. Hox transcription factors are crucial in the development and differentiation of neuronal fates by integrating spatial and sexual cues and are required for specifying both the positional and sexual fate. Studies of Hox family genes in C. elegans have established that neurons in the male are separated into three zones along the anterior to posterior axis. Two Hox transcription factors that belong to a subset of neurons in the ventral nerve cord, LIN-39 and MAB-5, are required to specify neurons in these 3 zones. Previous studies have established that a mutant form of LIN-39, LIN-39(ccc16), fails to specify cell fates only in the presence of MAB-5, but can function normally when MAB-5 is not present. This mutation has been located in the 5-exon splice acceptor, causing two alternative splice sites to be more favorable, both of which truncate the amino acids of the resulting protein. Determining structural differences between LIN-39 and its mutant while in the apo state and various bound states will guide our understanding of the molecular mechanisms utilized by Hox transcription factors to determine neuronal fate. Preliminary 15N-1H HSQC analysis and structure prediction tools indicate both apo and DNA bound LIN-39 adopt non-uniform, highly disordered and dynamic structures. These studies represent the first structural studies conducted on LIN-39 and offer insight to the contribution of intrinsically disordered states in gene regulation mechanisms.