A comprehensive set of measurements and calculated metrics describing physical, chemical, and biological conditions in the river corridor is presented. These data were collected in a catchment-wide, synoptic campaign in the H. J. Andrews Experimental Forest (Cascade Mountains, Oregon, USA) in summer 2016 during low-discharge conditions. Extensive characterization of 62 sites including surface water, hyporheic water, and streambed sediment was conducted spanning 1st- through 5th-order reaches in the river network. The objective of the sample design and data acquisition was to generate a novel data set to support scaling of river corridor processes across varying flows and morphologic forms present in a river network. The data are available at https://doi.org/10.4211/hs.f4484e0703f743c696c2e1f209abb842 (Ward, 2019).