Evidence-based practice (EBP) knowledge among prelicensure nursing students was measured before, immediately following, and 1 year after completion of an EBP course using a relatively new instrument. There was a significant increase in EBP knowledge immediately following the course, and knowledge was sustained 1 year later. Results enabled faculty to gauge the effectiveness of the EBP course within the curriculum to prepare students with the knowledge needed to enact EBP in practice.