Data from eighteen different truck washes were used to compare the association of different service levels with Enterobacteriaceae counts. Service levels were classified into three different categories; prewash (n=78), post wash with disinfectant (n=78), and post wash without disinfectant (n=12). A total of 168 drag swabs were used for collection for the purpose of this study. Prewash services were defined as trailers before they were scraped out and washed. Post wash with or without disinfectant services were defined as after the trailers were washed and disinfectant was or was not applied. Prewash trailers tended to have higher Enterobacteriaceae counts of around 5.0 Log10CFU/m2when compared to post wash with disinfectantEnterobacteriaceae counts of around 2.2 Log10CFU/m2 and post wash without disinfectant Enterobacteriaceae counts of around 2.7 Log10CFU/m2.