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Article
Determination of Alfentanil in Human Plasma Using Gas Liquid Chromatography with Capillary Column and Nitrogen Selective Detection
Asia Pacific Journal of Pharmacology (1988)
  • Kelvin Chan
Abstract

A gas liquid chromatography (GLC) for the determination of alfentanil in plasma of surgical patients is described. The assay involved a preliminary ethereal extraction of alfentanil and fentanyl (as internal standard) in a 0.5 ml plasma sample under alkaline conditions. The dried extract was dissolved in n-heptane (10 µl) and 1µl-aliquot was analysed by a Varian GLC system consisting of a SE-30 capillary column (16 m x 0.25 mm) linked to a nitrogen selective detector. The recovery of alfentanil from plasma samples at 250 ng ml-1 ranged from 84 to 92% (n = 10, mean 88%) with a coefficient of variation of 2.7%. Calibration graphs, over the concentration range of 5 to 500 ng ml-1, relating the peak height ratios of alfentanil to the internal standard were linear (n = 6; coefficients of variation ranging from 2.4 to 5.7%). The assay was used to measure the plasma concentrations of alfentanil in 4 surgical patients during and after its intravenous infusion and when spontaneous ventilation was re-established at the end of surgery.

Keywords
  • Alfentanil,
  • human plasma,
  • gas liquid chromatography,
  • capillary column
Publication Date
1988
Citation Information
Kelvin Chan. "Determination of Alfentanil in Human Plasma Using Gas Liquid Chromatography with Capillary Column and Nitrogen Selective Detection" Asia Pacific Journal of Pharmacology Vol. 3 (1988)
Available at: http://works.bepress.com/kelvin_chan/70/