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The use of a packed column for the determination of bupivacaine in human plasma by gas chromatography: an application in a pharmacokinetic study of bupivacaine
Forensic Science International (1992)
  • Kelvin Chan
Abstract

The method involves a single extraction of the drug and the internal standard, etidocaine, from alkalinized plasma with n-hexane. The gas chromatograph is equipped with a glass column (2.0 m x 2 mm) packed with 3% W/W SP2250 on Chromosorb W, (80/100 mesh) and a nitrogen sensitive detector. The method can accurately measure plasma bupivacaine concentrations down to 0.01 µg ml-1 using a sample size of 0.5 ml. The day-to-day variation of bupivacaine at 2.0 µg ml-1 is 6.90% (n = 10). The calibration graph is linear over the range 0.1 - 4.0 µg ml-1. The method is accurate, fast and sensitive and has been applied in a pharmacokinetic study of bupivacaine.

Keywords
  • bupivacaine,
  • human plasma,
  • single-step extraction,
  • n-Hexane,
  • gas chromatography,
  • packed column,
  • pharmacokinetic study
Publication Date
1992
Citation Information
Kelvin Chan. "The use of a packed column for the determination of bupivacaine in human plasma by gas chromatography: an application in a pharmacokinetic study of bupivacaine" Forensic Science International Vol. 53 (1992)
Available at: http://works.bepress.com/kelvin_chan/113/