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Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells
Federation of European Biochemical Societies Letters (2006)
  • Kelvin Chan
Abstract

We here provide definitive evidence that ginsenoside- Rg1, the pharmacologically active component of ginseng, is a functional ligand of the glucocorticoid receptor (GR) as determined by fluorescence polarization assay. Rg1 increased the phosphorylation of GR, phosphatidylinositol-3 kinase (PI3K), Akt/PKB and endothelial nitric oxide synthase (eNOS) leading to increase nitric oxide (NO) production in human umbilical vein endothelial cell. Rg1-induced eNOS phosphorylation and NO production were significantly reduced by RU486, LY294,002, or SH-6. Also, knockdown of GR completely eliminated the Rg1-induced NO production. This study revealed that Rgl can indeed serve as an agonist ligand for GR and the activated GR can induce rapid NO production from eNOS via the non-transcriptional PI3K/Akt pathway.

Keywords
  • Ginsenoside-Rg1,
  • Glucocorticoid receptor,
  • Nitric oxide,
  • endothelial cells
Publication Date
2006
Citation Information
Kelvin Chan. "Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells" Federation of European Biochemical Societies Letters Vol. 580 (2006)
Available at: http://works.bepress.com/kelvin_chan/112/