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Article
Peroxiredoxin II Regulates Effector and Secondary Memory CD8+ T cell Responses
Journal of Virology
  • Ryan D. Michalek, Metabolon Corporation
  • Katie E. Crump, Wake Forest University School of Medicine
  • Ashley E. Weant, Wake Forest University School of Medicine
  • Elizabeth M. Hiltbold, Wake Forest University School of Medicine
  • Daniel G. Juneau, Wake Forest University School of Medicine
  • Eun-Yi Moon, Sejong University
  • Dae-Yeul Yu, Korea Research Institute of Bioscience and Biotechnology
  • Leslie B. Poole, Wake Forest University School of Medicine
  • Jason M. Grayson, Wake Forest University School of Medicine
Document Type
Article
Publication Date
12-1-2012
Disciplines
Abstract
Reactive oxygen intermediates (ROI) generated in response to receptor stimulation play an important role in cellular responses. However, the effect of increased H2O2on an antigen-specific CD8+ T cell response was unknown. Following T cell receptor (TCR) stimulation, the expression and oxidation of peroxiredoxin II (PrdxII), a critical antioxidant enzyme, increased in CD8+ T cells. Deletion of PrdxII increased ROI, S phase entry, division, and death during in vitro division. During primary acute viral and bacterial infection, the number of effector CD8+ T cells in PrdxII-deficient mice was increased, while the number of memory cells were similar to those of the wild-type cells. Adoptive transfer of P14 TCR transgenic cells demonstrated that the increased expansion of effector cells was T cell autonomous. After rechallenge, effector CD8+ T cells in mutant animals were more skewed to memory phenotype than cells from wild-type mice, resulting in a larger secondary memory CD8+ T cell pool. During chronic viral infection, increased antigen-specific CD8+ T cells accumulated in the spleens of PrdxII mutant mice, causing mortality. These results demonstrate that PrdxII controls effector CD8+ T cell expansion, secondary memory generation, and immunopathology.
DOI
10.1128/JVI.01559-12
Citation Information
Ryan D. Michalek, Katie E. Crump, Ashley E. Weant, Elizabeth M. Hiltbold, et al.. "Peroxiredoxin II Regulates Effector and Secondary Memory CD8+ T cell Responses" Journal of Virology Vol. 86 Iss. 24 (2012) p. 13629 - 13641 ISSN: 0022-538X
Available at: http://works.bepress.com/katie-crump/6/