This paper presents two studies (qualitative and quantitative) with the shared goal of exploring preservice teachers’ (PSTs’) experiences of mathematics anxiety and self-efficacy for mathematics teaching. Findings indicate that PSTs experience high levels of mathematics anxiety, impacting current learning and preference for teaching the content, as well as the development of self-efficacy for teaching mathematics and conceptions of ideal teaching. Findings regarding anxiety (fear) of evaluation and concern about being able to inspire students in their future classrooms converged across studies.