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Systems-based analysis of Arabidopsis leaf growth reveals adaptation to water deficit
Molecular Systems Biology (2012)
  • Katja Baerenfaller, ETH Zurich
  • Catherine Massonnet, Laboratoire d’Ecophysiologie des Plantes sous Stress Environnementaux
  • Sean Walsh, ETH Zurich
  • Sacha Baginsky, ETH Zurich
  • Peter Buhlmann, ETH Zurich
  • Lars Hennig, ETH Zurich
  • Matthias Hirsch-Hoffmann, ETH Zurich
  • Kate Howell, The University of Notre Dame Australia
  • Sabine Kahlau, The University of Western Australia
  • Amandine Radziejwoski, Vlaams Instituut voor Biotechnologie
  • Doris Russenberger, ETH Zurich
Abstract
Leaves have a central role in plant energy capture and carbon conversion and therefore must
continuously adapt their development to prevailing environmental conditions. To reveal the dynamic
systems behaviour of leaf development, we profiled Arabidopsis leaf number six in depth at four
different growth stages, at both the end-of-day and end-of-night, in plants growing in two controlled
experimental conditions: short-day conditions with optimal soil water content and constant reduced
soil water conditions. We found that the lower soil water potential led to reduced, but prolonged,
growth and an adaptation at the molecular level without a drought stress response. Clustering of the
protein and transcript data using a decision tree revealed different patterns in abundance changes
across the growth stages and between end-of-day and end-of-night that are linked to specific
biological functions. Correlations between protein and transcript levels depend on the time-of-day
and also on protein localisation and function. Surprisingly, only very few of 41700 quantified
proteins showed diurnal abundance fluctuations, despite strong fluctuations at the transcript level.
Disciplines
Publication Date
2012
DOI
10.1038/msb.2012.39
Citation Information
Baerenfaller K*, Massonnet C*, Walsh S*, Baginsky S, Bühlmann P, Hennig L, Hirsch-Hoffmann M, Howell KA, Kahlau S, Radziejwoski A, Russenberger D, Rutishauser D, Small I, Stekhoven D, Sulpice R, Svozil J, Wuyts N, Stitt M, Hilson P, Granier C, Gruissem W (2012) Systems-based analysis of Arabidopsis leaf growth reveals adaptation to water deficit. Mol Syst Biol, 8: 606