The purpose of this study was to investigate cross-level interactions among subordinate and supervisor characteristics on subordinates’ work-family conflict. It was hypothesized that subordinates’ job demands, job control (autonomy, workgroup control, task and routine control) and support (organizational support and supervisor support) would be related to subordinates’ reports of work-family conflict. It was further hypothesized that supervisor characteristics (supervisor’ job demands, supervisors’ job control, supervisors’ managers’ support for supervisors’ work-family issues and supervisors’ organizational support for supervisors’ work-family issues) would be related to the relationship between subordinates’ supervisor support for subordinates’ work-family issues and subordinates’ work-family conflict. One hundred and eighty-seven employees working in a manufacturing plant in the Midwest and their 28 supervisors were surveyed. Intra-class correlation (ICC) was found to be less than 1% for the outcome variable. This indicated that much of the variation in the outcome variable, work-family conflict, was due to individual-level characteristics. Organizational Support and task and routine control had a negative and significant relationship with work-family conflict as reported by hourly wage earners working in a manufacturing plant.