Pumping of high-strength concrete (HSC) in high-rise or long-span structures is of paramount importance in constructions. However, pumping technology in practice is highly dependent on practical field experience, and full-scale testing that is carried out for complex projects involving new materials or long pumping distances. In this paper, a long pumping circuit was built to study changes in fresh properties of HSC due to pumping. In total, 10 HSC mixtures with strength grades of C60 to C100 were pumped at constant flow rates in pipelines measuring 348, 600 and 924 m in length. Rheological properties, workability characteristics, and concrete temperature were measured on concrete specimens sampled before and after pumping. Rheological properties of concrete sampled in dismantled pipes shortly after the end of pumping were also determined. Test results indicated that the yield stress increased, the initial tangential viscosity decreased and the shear-thickening phenomenon was eliminated due to pumping. The increase in yield stress mostly occurred in the first half of the pipeline, and the tangential viscosity decreased nearly linearly along the pipe length. The levels of drop in initial tangential viscosity were found to positively correspond to the calculated shearing energy, which is estimated given the shear rate, apparent viscosity and elapsed time during agitation and pumping.