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Local Efforts to Expand the World’s Largest Trichomycete Collection
College of Arts and Sciences Poster Presentations
  • Justin W. Gause, Department of Biological Sciences, Boise State University
  • Emma Wilson, Department of Biological Sciences, Boise State University
  • Nicole Reynolds, Department of Biological Sciences, Boise State University
  • Luke Sugden, Department of Biological Sciences, Boise State University
  • Alison Chamberlin, Department of Biological Sciences, Boise State University
  • Merlin White, Department of Biological Sciences, Boise State University
Document Type
Student Presentation
Presentation Date
4-16-2012
Faculty Sponsor
Merlin White
Abstract

Trichomycetes (gut fungi) are obligate symbionts of various arthropods and have been found in marine, freshwater, and terrestrial habitats on every continent except Antarctica. Gut fungi associate commensally with their immature aquatic hosts (including black flies, mayflies, stoneflies, isopods, and others) attaching to the chitinous lining of the mid- or hindgut. Gut fungi are routinely isolated from the host’s digestive tract into axenic cultures, which provide a rich resource of genetic material for phylogenic analysis. Given the obligate endosymbiotic nature of the fungi, unique challenges are encountered upon culturing, thus only 30% of all trichomycetes have been successfully cultured. Our laboratory houses the world’s largest culture collection of trichomycetes. We have been endeavoring to expand the collection by formulating suitable media in which field collected trichomycete specimens can grow. This requires attention to variables such as competing bacterial growth, pH changes required for sporulation, agar density and proper nutrient balance within the media. The continued development and enhancement of culture media is intended to increase the number of isolates that previously were considered "unculturable" and offer more robust samples of genetic material for future analyses.

Citation Information
Justin W. Gause, Emma Wilson, Nicole Reynolds, Luke Sugden, et al.. "Local Efforts to Expand the World’s Largest Trichomycete Collection" (2012)
Available at: http://works.bepress.com/justin_gause/3/