The mobile industry processor interface (MIPI) standards defines industry specification for design of mobile devices such as smartphone, tables, laptop and hybrid devices. Such standard plays a critical role in the Internet of Things (IoT), 5G mobile devices, as well as autopilot automobiles. MIPI specification has comprehensive requirements on the data storage, data transfer, display, camera, memory, power, etc on the transmitter and receiver. In the testing phase, the transmitter and receiver may not be easily directly measured. Herein, a fast transmitter and receiver eye diagrams calculation methodology is necessary to predict the RX and TX performances of those devices. The calculated RX and TX eye diagrams are evaluated by using the D-PHY protocol to identify the quality of the signals. An in-house MIPI tool is built to directly display the calculated eye diagrams as well as the MIPI D-PHY report in the PC. The validations are performed by using both simulation and measurement examples.