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Selected Works of Julian Tyson
Article
Characterization of selenium species in biological extracts by enhanced ion-pair liquid chromatography with inductively coupled plasma-mass spectrometry and by referenced electrospray ionization-mass spectrometry
Spectrochimica Acta Part B (1999)
  • Julian Tyson
  • Mihaly Kotrebai
  • Susan M Bird
  • Eric Block
  • Peter C Uden
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Abstract
Selenium is an essential nutrient for humans; selenium compounds catalyze intermediate metabolism reactions
and inhibit the toxic effects of heavy metals such as arsenic, cadmium and mercury. Some extracts of seleniumenriched
biological materials show cancer preventive effects, tentatively attributable to the biological functions of
selenoamino acids. An improved ion pair chromatographic method with methodological enhancements for the
separation, qualitative and quantitative determination of non-volatile selenium compounds extracted from different
samples has been developed using ICP-MS as an element-selective detector. Separation power early in the
chromatogram was increased to baseline separation in the standard mixture as a result of decreasing spray chamber
size from 97 to 14 ml, and increasing trifluoracetic acid TFA. concentration in the mobile phase from 0.1 to 0.6%.
The former pH was restored by the addition of ammonia to the mobile phase, which also served to increase the
column recovery of inorganic anions. Calibration curves for different selenoamino acids showed statistically different
behavior. Biological sample extracts were characterized using HPLC-ICP-MS. Mass spectral behavior of selenoamino acids,
using electrospray and ion trap technology with direct infusion and liquid chromatographic sample introduc-tion, is also
reported.
Keywords
  • Selenoamino acids,
  • Speciation,
  • High performance liquid chromatography,
  • Inductively coupled plasma-mass spectrometry,
  • Electrospray ionization-mass spectrometry
Disciplines
Publication Date
1999
Citation Information
Julian Tyson, Mihaly Kotrebai, Susan M Bird, Eric Block, et al.. "Characterization of selenium species in biological extracts by enhanced ion-pair liquid chromatography with inductively coupled plasma-mass spectrometry and by referenced electrospray ionization-mass spectrometry" Spectrochimica Acta Part B Vol. 54 (1999) p. 1573 - 1591
Available at: http://works.bepress.com/julian_tyson/25/