Article
Analytical selenoamino acid studies by chromatography with interfaced atomic mass spectrometry and atomic emission spectral detection
Fresenius' Journal of Analytical Chemistry
(1998)
Abstract
Consumption of selenium enriched plants or
yeast-based nutritional supplements has been reported to
provide anticarcinogenic benefits which are selenium
compound dependent. Separation and identification of
these selenium compounds is critical to understand the activity.
Plants and yeast convert inorganic selenium in the
soil or growth media into organoselenium compounds,
probably following a route similar to the sulfur assimilatory
pathway. Non-volatile selenium compounds produced
include selenoamino acids, some of which have
shown anticarcinogenic activity. Volatile compounds produced
by chemical reaction of involatile precursors have
also been found. An ion pair chromatographic method
with ICP-MS detection for the separation of selenoamino
acid standards potentially present in real samples is given.
The method allows separation of selenoamino acids including
such analytes as the cis-trans isomers of Se-1-
propenyl-DL-selenocysteine. The method also provides
the capability of determining the presence of selenoxides
and possibly selenones, and tracking of other functionalities
and reactions by selective derivatization. Alternatively,
selenoamino acids are treated with ethylchloroformate
to produce stable volatile derivatives which are
amenable to GC separation with element specific atomic
emission detection (GC-AED). Results of total selenium
determination and speciation of selenium enriched yeastbased
nutritional supplements, selenium enriched allium
vegetables and bioremediation samples are presented.
Keywords
- Chemistry,
- selenoamino acids,
- chromatography,
- interfaced atomic mass spectrometry,
- atomic emission spectral detection
Disciplines
Publication Date
1998
Citation Information
Julian Tyson, Peter C Uden, Susan M Bird, Mihaly Kotrebai, et al.. "Analytical selenoamino acid studies by chromatography with interfaced atomic mass spectrometry and atomic emission spectral detection" Fresenius' Journal of Analytical Chemistry Vol. 362 (1998) p. 447 - 456 Available at: http://works.bepress.com/julian_tyson/18/