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Article
Potential Use of a Terbium-Transferrin Complex as a Label in an Immunoassay for Gentamicin
Analyst (1984)
  • Julian Tyson
  • Nichola J. Wilmott
  • James N. Miller
Abstract
A study has been made of the potential use of a terbium - transferrin complex as a non-isotopic label in the
immunoassay determination of the antibiotic gentamicin. The fluorescence properties of the complex have
been characterised. The labelled gentamicin was formed using a controlled carbodiimide reaction, conditions
being chosen to produce a gentamicin-bound complex containing the correct amount of gentamicin for use in
a competitive binding assay. Recognition of the gentamicin-bound complex by antisera to gentamicin was
verified using a standard radioimmunoassay for gentamicin.
Keywords
  • Fluorescence immunoassay,
  • gentamicin assay,
  • terbium-transferrin label
Publication Date
1984
Citation Information
Julian Tyson, Nichola J. Wilmott and James N. Miller. "Potential Use of a Terbium-Transferrin Complex as a Label in an Immunoassay for Gentamicin" Analyst Vol. 109 (1984) p. 343
Available at: http://works.bepress.com/julian_tyson/145/