Article
High Performance Liquid Chromatography of selenoaminoacids and Organo-selenium Compounds. Speciation by inductively coupled plasma mass spectrometry.
Journal of Chromatography A
(1997)
Abstract
As part of an ongoing study to identify selenium compounds with cancer chemopreventive activity, extracts of
selenium-enriched samples were analyzed by HPLC–inductively coupled plasma (ICP)-MS. Ion-exchange, ion pair and
derivatization methods for reversed-phase HPLC were considered and advantages and disadvantages for each compared.
Anion exchange allows separation of selenite and selenate, but otherwise provides poor separation. Pre-column derivatization
and reversed-phase chromatography provides separation of compounds with terminal amine functionalities, but many other
species elute in the void volume. The ion pair method gave optimal separation and was compatible with standard ICP-MS
operating conditions.
Keywords
- Selenoamino acids,
- Selenium,
- Organoselenium compounds
Disciplines
Publication Date
1997
Citation Information
Julian Tyson, Susan M. Bird, Honghong Ge, Peter C. Uden, et al.. "High Performance Liquid Chromatography of selenoaminoacids and Organo-selenium Compounds. Speciation by inductively coupled plasma mass spectrometry." Journal of Chromatography A Vol. 789 (1997) p. 349 - 359 Available at: http://works.bepress.com/julian_tyson/108/