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Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation
Biochemistry and Microbiology
  • David J. Kennedy
  • Sandeep Vetteth
  • Miaorong Xie
  • Sankaridrug M. Periyasamy
  • Jingwei Xie, Marshall University
  • Chi Han
  • Venkatesha Basrur
  • Krishna Mutgi
  • Vladimir Fedorov
  • Deepak Malhotra
  • Joseph I. Shapiro, M.D., Marshall University
Document Type
Article
Publication Date
7-17-2006
Abstract

The effect of cardiac glycosides to increase cardiac inotropy by altering Ca2+ cycling is well known but still poorly understood. The studies described in this report focus on defining the effects of ouabain signaling on sarcoplasmic reticulum Ca2+-ATPase function. Rat cardiac myocytes treated with 50 μM ouabain demonstrated substantial increases in systolic and diastolic Ca2+ concentrations. The recovery time constant for the Ca2+ transient, τ, was significantly prolonged by ouabain. Exposure to 10 μM H2O2, which causes an increase in intracellular reactive oxygen species similar to that of 50 μM ouabain, caused a similar increase in τ. Concurrent exposure to 10 mM N-acetylcysteine or an aqueous extract from green tea (50 mg/ml) both prevented the increases in τ as well as the changes in systolic or diastolic Ca2+ concentrations. We also observed that 50 μM ouabain induced increases in developed pressure in addition to diastolic dysfunction in the isolated perfused rat heart. Coadministration of ouabain with N-acetylcysteine prevented these increases. Analysis of sarcoplasmic reticulum Ca2+-ATPase protein revealed increases in both the oxidation and nitrotyrosine content in the ouabain-treated hearts. Liquid chromatography-mass spectrometric analysis confirmed that the sarcoplasmic reticulum Ca2+-ATPase protein from ouabain-treated hearts had modifications consistent with oxidative and nitrosative stress. These data suggest that ouabain induces oxidative changes of the sarcoplasmic reticulum Ca2+-ATPase structure and function that may, in turn, produce some of the associated changes in Ca2+ cycling and physiological function.

Comments

The copy of record is available from the publisher at http://ajpheart.physiology.org/content/ajpheart/291/6/H3003.full. Copyright © 2006 the American Physiological Society. doi: 10.1152/ajpheart.00603.2006

Citation Information
Kennedy DJ, Vetteth S, Xie M, Periyasamy SM, Xie Z, Han C, Basrur V, Mutgi K, Fedorov V, Malhotra D, and Shapiro JI (2006b) Ouabain decreases sarco(endo)-plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation. Am J Physiol Heart Circ Physiol 291: H3003-H3011.