Article
Improved Sensitivity and Specificity for Trans-Resveratrol in Red Wine Analysis with HPLC–UV and LC–MS
LCGC North America
(2016)
Abstract
Adequate detection of trans-resveratrol in wine is complicated by two factors: relatively low levels and interferences from matrix components. Here, we present two useful approaches to overcoming these issues depending on the instrumentation available. For high performance liquid chromatography (HPLC) analyses with ultraviolet (UV) detection, matrix peaks can be removed by microextraction using a packed sorbent while simultaneously concentrating the trans-resveratrol peak by a factor of two. For liquid chromatography-mass spectrometry (LC–MS), the extracted ion chromatogram for the [M + H]+ analyte ion can be used to obtain specificity without prior extraction procedures.
Disciplines
Publication Date
2016
Citation Information
Joshua E. Young, Michael V. Lim, Joshua Topete, Hao Hang, et al.. "Improved Sensitivity and Specificity for Trans-Resveratrol in Red Wine Analysis with HPLC–UV and LC–MS" LCGC North America Vol. 34 Iss. 3 (2016) p. 206 - 213 Available at: http://works.bepress.com/joseph_pesek/380/