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Assessing sequential oncogene amplification in human breast cancer
Cytometry (1995)
  • Laura E. Janocko
  • Joseph F. Lucke, State University of New York at Buffalo
  • David W. Groft
  • Kathryn A. Brown
  • Charles A. Smith
  • Agnese A. Pollice
  • Sarita G. Singh
  • Robert Yakulis
  • Robert J. Hartsock
  • Stanley E. Shackney

Studies of amplification and/or overexpression of c-myc, HER-2/neu, and H-ras in breast cancer have shown that each is associated with a poor prognosis. The purpose of this study was to explore the possibility that there is a preferred sequence of amplification of these oncogenes in breast cancer. The frequencies of amplification and patterns of co-amplification of c-myc, HER-2/neu, and H-ras were studied in a group of 84 breast cancers. The data suggested a preferred sequence of amplification that consisted of c-myc amplification-HER-2/neu amplification-H-ras amplification. This model was supported by loglinear analysis. In addition, the levels of amplification of JC-A, a DNA fragment newly isolated from a patient with advanced breast cancer, were studied in 61 of these cases. The data suggested that JC-A amplification occurred early. Loglinear analysis supported a model in which JC-A amplification occurred either before or after c-myc amplification but was unrelated to Her-2/neu or ras amplification.

  • Oncogene amplification; oncogene overexpression; breast cancer; genetic evolution; log-linear statistical models;
Publication Date
Citation Information
Laura E. Janocko, Joseph F. Lucke, David W. Groft, Kathryn A. Brown, et al.. "Assessing sequential oncogene amplification in human breast cancer" Cytometry Vol. 21 Iss. 1 (1995)
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