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Article
Assessing sequential oncogene amplification in human breast cancer
Cytometry (1995)
  • Laura E. Janocko
  • Joseph F. Lucke, State University of New York at Buffalo
  • David W. Groft
  • Kathryn A. Brown
  • Charles A. Smith
  • Agnese A. Pollice
  • Sarita G. Singh
  • Robert Yakulis
  • Robert J. Hartsock
  • Stanley E. Shackney
Abstract

Studies of amplification and/or overexpression of c-myc, HER-2/neu, and H-ras in breast cancer have shown that each is associated with a poor prognosis. The purpose of this study was to explore the possibility that there is a preferred sequence of amplification of these oncogenes in breast cancer. The frequencies of amplification and patterns of co-amplification of c-myc, HER-2/neu, and H-ras were studied in a group of 84 breast cancers. The data suggested a preferred sequence of amplification that consisted of c-myc amplification-HER-2/neu amplification-H-ras amplification. This model was supported by loglinear analysis. In addition, the levels of amplification of JC-A, a DNA fragment newly isolated from a patient with advanced breast cancer, were studied in 61 of these cases. The data suggested that JC-A amplification occurred early. Loglinear analysis supported a model in which JC-A amplification occurred either before or after c-myc amplification but was unrelated to Her-2/neu or ras amplification.

Keywords
  • Oncogene amplification; oncogene overexpression; breast cancer; genetic evolution; log-linear statistical models;
Publication Date
1995
Citation Information
Laura E. Janocko, Joseph F. Lucke, David W. Groft, Kathryn A. Brown, et al.. "Assessing sequential oncogene amplification in human breast cancer" Cytometry Vol. 21 Iss. 1 (1995)
Available at: http://works.bepress.com/joseph_lucke/32/