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Article
Cryptosporidium Parvum-Induced Inflammatory Bowel Disease of TCR-β- x TCR-δ-Deficient Mice
The Journal of Parasitology
  • W. Ray Waters, Iowa State University
  • Michael J. Wannemeuhler, Iowa State University
  • Randy E. Sacco, U.S. Department of Agriculture
  • Mitchell V. Palmer, U.S. Department of Agriculture
  • Joseph S. Haynes, Iowa State University
  • Bruce A Pesch, U.S. Department of Agriculture
  • James A Harp, U.S. Department of Agriculture
Document Type
Article
Publication Version
Published Version
Publication Date
12-1-1999
DOI
10.2307/3285674
Abstract
Experimental inoculation of neonatal immunocompetent strains of mice with Cryptosporidium parvum results in a transient, noninflammatory enteric infection. In the present study, we show that inoculation of mice deficient in a 3 and y8 T cells (TCR-3- X TCR-8-deficient mice) with C. parvum results in persistent infection and severe inflammatory bowel disease- like lesions. The most severe lesions in these mice were in the cecum with similar yet less severe lesions in the ileum and proximal colon. The most notable aspect of the histopathology was glandular hyperplasia with abscess formation, extensive fibrosis of the lamina propria with infiltrates of predominately polymorphonuclear cells and macrophages, and a few small aggregates of B cells. Persistently infected mice also developed extensive hepatic periportal fibrosis in association with C. parvum colonization of bile ducts. Lesions observed in TCR- 3- X TCR-8-deficient mice were markedly different than previously described lesions detected in C. parvum-infected TCR-o-deficient mice. Cryptosporidium parvum-infected TCR-o-deficient mice have extensive infiltrations of B cells, whereas TCR-P3- X TCR-8-deficient mice had only a few small aggregates of B cells. These findings indicate that although y8 T cells are not necessary for induction of intestinal inflammation in C. parvum-infected o(4 T- cell-deficient mice, their presence does alter the morphology of the ensuing lesion.
Comments

This article is published as Waters, W. Ray, Michael J. Wannemuehler, Randy E. Sacco, Mitchell V. Palmer, Joseph S. Haynes, Bruce A. Pesch, and James A. Harp. "Cryptosporidium parvum-induced inflammatory bowel disease of TCR-β-x TCR-δ-deficient mice." The Journal of parasitology (1999): 1100-1105. 10.2307/3285674. Posted with permission.

Rights
Works produced by employees of the government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
W. Ray Waters, Michael J. Wannemeuhler, Randy E. Sacco, Mitchell V. Palmer, et al.. "Cryptosporidium Parvum-Induced Inflammatory Bowel Disease of TCR-β- x TCR-δ-Deficient Mice" The Journal of Parasitology Vol. 85 Iss. 6 (1999) p. 1100 - 1105
Available at: http://works.bepress.com/joseph-haynes/8/