Currently, there is an increased intake of omega-3 (n-3) polyunsaturated fatty acids (PUFA) through diet and supplementation. The objective of this study was to determine if the n-3 PUFA source plays a role in de novo lipid metabolism by altering gene expression. Female Sprague-Dawley rats were assigned to one of six diets: corn oil (CO), flaxseed oil (FO), krill oil (KO), menhaden oil (MO), salmon oil (SO), or tuna oil (TO). Most n −3 PUFA sources had decreased expression of hepatic lipogenic genes. Stearoyl CoA Desaturase 1 expression decreased in TO (P=0.04) and MO (P=0.008) compared to CO. Fatty Acid Synthase expression decreased in TO (P=0.006), SO (P=0.001), MO (P=0.001), and FO (P=0.01) compared to CO. Sterol Regulatory Element Binding Protein 1c expression decreased in MO (P=0.007) compared to CO. Peroxisome Proliferator-Activated Receptor Alpha (PPAR α) was used as a measure of hepatic lipolysis, however, no differences were found. There were no significant changes in expression of lipogenic genes in gonadal tissue of n-3 PUFA groups when compared to CO. In the gonadal tissue, PPARγ increased in FO (P=0.04) compared to CO, and Hormone Sensitive Lipase had no differences in expression compared to CO. These values suggest that n-3 fatty acids may be altering de novo lipid metabolism by decreasing expression of lipogenic genes in liver tissue, and increasing expression of a lipolytic gene in gonadal tissue.