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Method for somatic cell nuclear transfer in zebrafish
Methods Cell Biology (2011)
  • Kannika Siripattarapravat
  • Jose CIbelli
Abstract

Somatic cell nuclear transfer (SCNT) has been a well-known technique for decades and widely applied to generate identical animals, including ones with genetic alterations. The system has been demonstrated successfully in zebrafish. The elaborated requirements of SCNT, however, limit reproducibility of the established model to a few groups in zebrafish research community. In this chapter, we meticulously outline each step of the published protocol as well as preparations of equipments and reagents used in zebrafish SCNT.

Publication Date
2011
Citation Information
Kannika Siripattarapravat and Jose CIbelli. "Method for somatic cell nuclear transfer in zebrafish" Methods Cell Biology (2011)
Available at: http://works.bepress.com/jose_cibelli/3/