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Oscillatory glucose flux in INS 1 pancreatic β cells: A self-referencing microbiosensor study
Analytical Biochemistry (2011)
  • Jin Shi, Purdue University
  • Eric S. McLamore, Purdue University
  • David B. Jaroch, Purdue University
  • Jonathan C. Claussen, Purdue University
  • Raghavendra G. Mirmira, Indiana University School of Medicine
  • Jenna L. Rickus, Purdue University
  • D. Marshall Porterfield, Purdue University
Signaling and insulin secretion in β cells have been reported to demonstrate oscillatory modes, with abnormal oscillations associated with type 2 diabetes. We investigated cellular glucose influx in β cells with a self-referencing (SR) microbiosensor based on nanomaterials with enhanced performance. Dose–response analyses with glucose and metabolic inhibition studies were used to study oscillatory patterns and transporter kinetics. For the first time, we report a stable and regular oscillatory uptake of glucose (averaged period 2.9 ± 0.6 min), which corresponds well with an oscillator model. This oscillatory behavior is part of the feedback control pathway involving oxygen, cytosolic Ca2+/ATP, and insulin secretion (periodicity approximately 3 min). Glucose stimulation experiments show that the net Michaelis–Menten constant (6.1 ± 1.5 mM) is in between GLUT2 and GLUT9. Phloretin inhibition experiments show an EC50 value of 28 ± 1.6 μM phloretin for class I GLUT proteins and a concentration of 40 ± 0.6 μM phloretin caused maximum inhibition with residual nonoscillating flux, suggesting that the transporters not inhibited by phloretin are likely responsible for the remaining nonoscillatory uptake, and that impaired uptake via GLUT2 may be the cause of the oscillation loss in type 2 diabetes. Transporter studies using the SR microbiosensor will contribute to diabetes research and therapy development by exploring the nature of oscillatory transport mechanisms.

  • Glucose oxidase,
  • Biosensor,
  • Self-referencing,
  • Diabetes,
  • Glycolysis,
  • β Cell
Publication Date
April 15, 2011
Publisher Statement
This is a manuscript of an article published as Shi, Jin, Eric S. McLamore, David Jaroch, Jonathan C. Claussen, Raghavendra G. Mirmira, Jenna L. Rickus, and D. Marshall Porterfield. "Oscillatory glucose flux in INS 1 pancreatic β cells: A self-referencing microbiosensor study." Analytical Biochemistry 411, no. 2 (2011): 185-193. Posted with permission.

Copyright 2010 Elsevier Inc.

Citation Information
Jin Shi, Eric S. McLamore, David B. Jaroch, Jonathan C. Claussen, et al.. "Oscillatory glucose flux in INS 1 pancreatic β cells: A self-referencing microbiosensor study" Analytical Biochemistry Vol. 411 Iss. 2 (2011) p. 185 - 193
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