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Presentation
Impedance biosensor for the rapid detection of Listeria spp. based on aptamer functionalized Pt-interdigitated microelectrodes array
SPIE Commercial + Scientific Sensing and Imaging, 2016 (2016)
  • R. Sidhu, Texas A&M University
  • Y. Rong, University of Florida
  • D. C. Vanegas, University del Valle
  • Jonathan Claussen, Iowa State University
  • E. S. McLamore, University of Florida
  • Carmen L. Gomes, Iowa State University
Abstract
Listeria monocytogenes is one of the most common causes of food illness deaths worldwide, with multiple outbreaks in the United States alone. Current methods to detect foodborne pathogens are laborious and can take several hours to days to produce results. Thus, faster techniques are needed to detect bacteria within the same reliability level as traditional techniques. This study reports on a rapid, accurate, and sensitive aptamer biosensor device for Listeria spp. detection based on platinum interdigitated array microelectrodes (Pt-IDEs). Pt-IDEs with different geometric electrode gaps were fabricated by lithographic techniques and characterized by cyclic voltammetric (CV), electrochemical impedance spectroscopy (EIS), and potential amperometry (DCPA) measurements of reversible redox species. Based on these results, 50 μm Pt-IDE was chosen to further functionalize with a Listeria monocytogenes DNA aptamer selective to the cell surface protein internalin A, via metal-thiol self-assembly at the 5' end of the 47-mer's. EIS analysis was used to detect Listeria spp. without the need for label amplification and pre-concentration steps. The optimized aptamer concentration of 800 nM was selected to capture the bacteria through internalin A binding and the aptamer hairpin structure near the 3' end. The aptasensor was capable of detecting a wide range of bacteria concentration from 10 to 106 CFU/mL at lower detection limit of 5.39 ± 0.21 CFU/mL with sensitivity of 268.1 ± 25.40 (Ohms/log [CFU/mL]) in 17 min. The aptamer based biosensor offers a portable, rapid and sensitive alternative for food safety applications with one of the lowest detection limits reported to date.
Publication Date
May 13, 2016
Location
Baltimore, MD
DOI
10.1117/12.2223443
Comments
Copyright 2016 International Society for Optics and Photonics
Citation Information
R. Sidhu, Y. Rong, D. C. Vanegas, Jonathan Claussen, et al.. "Impedance biosensor for the rapid detection of Listeria spp. based on aptamer functionalized Pt-interdigitated microelectrodes array" SPIE Commercial + Scientific Sensing and Imaging, 2016 (2016) p. 98630F - 98630F
Available at: http://works.bepress.com/jonathan_claussen/35/