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Rapid and Label-Free Detection of Interferon Gamma via an Electrochemical Aptasensor Comprising a Ternary Surface Monolayer on a Gold Interdigitated Electrode Array
ACS Sensors
  • Shaowei Ding, Iowa State University
  • Curtis L. Mosher, Iowa State University
  • Xian Y. Lee, Iowa State University
  • Suprem R. Das, Iowa State University
  • Allison A. Cargill, Iowa State University
  • Xiaohui Tang, Iowa State University
  • Bolin Chen, Iowa State University
  • Eric S. McLamore, University of Florida
  • Carmen Gomes, Texas A & M University, College Station
  • Jesse M. Hostetter, Iowa State University
  • Jonathan C. Claussen, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-2017
DOI
10.1021/acssensors.6b00581
Abstract
A label-free electrochemical impedance spectroscopy (EIS) aptasensor for rapid detection (<35 min) of interferon-gamma (IFN-γ) was fabricated by immobilizing a RNA aptamer capture probe (ACP), selective to IFN-γ, on a gold interdigitated electrode array (Au IDE). The ACP was modified with a thiol group at the 5′ terminal end and subsequently co-immobilized with 1,6-hexanedithiol (HDT) and 6-mercapto-1-hexanolphosphate (MCH) to the gold surface through thiol–gold interactions. This ACP/HDT-MCH ternary surface monolayer facilitates efficient hybridization with IFN-γ and displays high resistance to nonspecific adsorption of nontarget proteins [i.e., fetal bovine serum (FBS) and bovine serum albumin (BSA)]. The Au IDE functionalized with ACP/HDT-MCH was able to measure IFN-γ in actual FBS solution with a linear sensing range from 22.22 pM to 0.11 nM (1–5 ng/mL) and a detection limit of 11.56 pM. The ability to rapidly sense IFN-γ within this sensing range makes the developed electrochemical platform conducive toward in-field disease detection of a variety of diseases including paratuberculosis (i.e., Johne’s Disease). Furthermore, experimental results were numerically validated with an equivalent circuit model that elucidated the effects of the sensing process and the influence of the immobilized ternary monolayer on signal output. This is the first time that ternary surface monolayers have been used to selectively capture/detect IFN-γ on Au IDEs.
Comments

Reprinted with permission from ACS Sensors 2 (2017): 210, doi:10.1021/acssensors.6b00581. Copyright 2017 American Chemical Society.

Copyright Owner
American Chemical Society
Language
en
File Format
application/pdf
Citation Information
Shaowei Ding, Curtis L. Mosher, Xian Y. Lee, Suprem R. Das, et al.. "Rapid and Label-Free Detection of Interferon Gamma via an Electrochemical Aptasensor Comprising a Ternary Surface Monolayer on a Gold Interdigitated Electrode Array" ACS Sensors (2017)
Available at: http://works.bepress.com/jonathan_claussen/29/