Article
Impact of flow rate, collection devices and extraction methods on tear concentrations following oral administration of doxycycline in dogs and cats
J Ocul Pharmacol Ther.
(2018)
Abstract
Purpose: Compare the precision of doxycycline quantification in tear fluid collected with either Schirmer strips or polyvinyl acetal (PVA) sponges following oral drug administration.
Methods: Three dogs and three cats were administered doxycycline orally at a dose of 4.2-5 mg/kg every 12 hours for 6 consecutive days. At day 5 and day 6, blood and tear fluid were sampled to capture doxycycline trough and maximal concentrations. Tear fluid was collected three times (spaced 10 minutes apart) at each session with the absorbent material placed in the lower conjunctival fornix until the 20-mm mark was reached (Schirmer strip, one eye) or for 1 min (PVA sponge, other eye). Tear extraction was performed with either centrifugation or elution in methanol. Doxycycline concentrations were measured with liquid chromatography-mass spectrometry. Low (100 ng/mL) and high (1000 ng/mL) tear concentrations measured in vivo were spiked into each absorbent material in vitro to evaluate percentage drug recovery.
Results: After oral administration of doxycycline, the drug reached the tear compartment at concentrations of 45.1-900.7 ng/mL in cats and 45.4-632.0 ng/mL in dogs, representing a tear-to-serum ratio of 12 % and 16%, respectively. Doxycycline tear concentrations were significantly more precise when tear collection was performed with Schirmer strips rather than PVA sponges (P = 0.007), but were not correlated with tear flow rate. In vitro doxycycline recovery was poor to moderate (< 75%).
Conclusions: Schirmer strips represent a good option for lacrimal doxycycline quantification, although the collection and subsequent extraction have to be optimized to improve drug recovery.
Disciplines
Publication Date
August, 2018
Citation Information
Jonathan P. Mochel. "Impact of flow rate, collection devices and extraction methods on tear concentrations following oral administration of doxycycline in dogs and cats" J Ocul Pharmacol Ther. (2018) Available at: http://works.bepress.com/jonathan-mochel/25/