The objective of this study was to determine the ability of a single bout of resistance exercise alone or in combination with recombinant human growth hormone (rhGH) to stimulate myofibrillar protein synthesis (Ks) in hindlimb suspended (HLS) adult female rats. Plantar flexor muscles were stimulated with resistance exercise, consisting of 10 repetitions of ladder climbing on a 1m grid (85 °), carrying an additional 50% of their body weight attached to their tails. Saline or rhGH (1 mg/kg) was administered 30′ prior to exercise, and Ks was determined with a constant infusion of 3H-Leucine at 15′, 60′, 180′, and 360′ following exercise. Three days of HLS depressed Ks 6̃5% and 30–40% in the soleus and gastrocnemius muscles, respectively (p