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Article
Modification of a commercial Toxoplasma gondii immunoglobulin G enzyme-linked immunosorbent assay for use in multiple animal species
Journal of Veterinary Diagnostic Investigation (2011)
  • John J Schaefer, University of Tennessee, Knoxville
  • Holly A White
  • Stephanie L Schaaf
  • Hussni O Mohammed
  • Susan E Wade
Abstract
A challenge faced by veterinary diagnosticians in serologic analysis for exposure to pathogens is the need for a protein conjugate capable of antibody attachment in many animal species. The advent of protein conjugates that are less specific in nature allows diagnosis across many species with little or no modification of technique. Toxoplasma gondii is an organism of veterinary interest that has been demonstrated to infect a plethora of warm-blooded animals. However, the serologic tests available for simultaneous diagnosis in this broad range are limited in number. The current study examined the use of an immunoglobulin G enzyme-linked immunosorbent assay (ELISA) modified by the use of non–species-specific protein conjugates in domestic animal species commonly submitted to diagnostic laboratories for evaluation of Toxoplasma exposure status. Comparison with results from an established indirect hemagglutination technique revealed very good agreement between the 2 test methods. This modification of the ELISA provides a useful method for veterinary diagnosticians to perform rapid and accurate evaluation of multiple animal species for Toxoplasma exposure using a single test. doi: 10.1177/104063871102300215
Keywords
  • Enzyme-linked immunosorbent assay,
  • protein A,
  • protein G,
  • serologic testing,
  • Toxoplasma gondii,
  • toxoplasmosis
Disciplines
Publication Date
2011
Citation Information
John J Schaefer, Holly A White, Stephanie L Schaaf, Hussni O Mohammed, et al.. "Modification of a commercial Toxoplasma gondii immunoglobulin G enzyme-linked immunosorbent assay for use in multiple animal species" Journal of Veterinary Diagnostic Investigation Vol. 23 Iss. 2 (2011)
Available at: http://works.bepress.com/john_schaefer/3/