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Article
Re-analysis of Magic Angle Spinning Nuclear Magnetic Resonance Determination of Interlamellar Waters in Lipid Bilayer Dispersions
Biophysical Journal
  • John F. Nagle, Carnegie Mellon University
  • Yufeng Liu, Carnegie Mellon University
  • Stephanie Tristram-Nagle, Carnegie Mellon University
  • Richard M. Epend, McMaster University
  • Ruth E. Stark, City University of New York
Date of Original Version
10-1-1999
Type
Article
Abstract or Description

A recent method to obtain the number of water molecules of hydration of multilamellar lipid vesicles using magic angle spinning nuclear magnetic resonance has been re-examined. The previous interpretation divided the water into bulk and interlamellar water and ignored water in defects (lakes) that are intrinsic to multilamellar lipid vesicles; the result was inconsistent with x-ray results for the lipid DOPC. The new interpretation takes advantage of the reduction of lake water with increased spinning and it uses osmotic pressure measurements to determine the loss of interlamellar water. The new result for DOPC from magic angle spinning is consistent with x-ray results.

DOI
10.1016/S0006-3495(99)77047-5
Citation Information
John F. Nagle, Yufeng Liu, Stephanie Tristram-Nagle, Richard M. Epend, et al.. "Re-analysis of Magic Angle Spinning Nuclear Magnetic Resonance Determination of Interlamellar Waters in Lipid Bilayer Dispersions" Biophysical Journal Vol. 77 Iss. 4 (1999) p. 2062 - 2065
Available at: http://works.bepress.com/john_nagle1/14/