Skip to main content
Article
Kinetic analysis of IgG antibodies to beta-amyloid oligomers with surface plasmon resonance
Analytical Biochemistry
  • Amanda C. Crisostomo
  • Loan Dang
  • Jyothi L. Digambaranath
  • Andrea C. Klaver
  • David A. Loeffler
  • Jeremiah J. Payne
  • Lynnae M. Smith
  • Adam L. Yokom
  • John M. Finke, University of Washington Tacoma
Publication Date
7-15-2015
Document Type
Article
Abstract

Surface plasmon resonance was used to investigate the kinetics, affinity, and specificity of binding between anti-Aβ (beta-amyloid) IgG antibodies and oligomeric Aβ. Two factors were needed to accurately characterize the IgG binding kinetics. First, a bivalent model was necessary to properly fit the kinetic association and dissociation sensograms. Second, a high concentration of IgG was necessary to overcome a significant mass transport limitation that existed regardless of oligomer density on the sensor surface. Using high IgG concentrations and bivalent fits, consistent kinetic parameters were found at varying sensor surface ligand densities. A comparison of binding specificity, affinity, and kinetic flux between monoclonal and natural human anti-Aβ IgG antibodies revealed the following findings. First, monoclonal antibodies 6E10 and 4G8 single-site binding affinity is similar between Aβ oligomers and monomers. Second, natural human anti-Aβ IgG binding readily binds Aβ oligomers but does not bind monomers. Third, natural human anti-Aβ IgG binds Aβ oligomers with a higher affinity and kinetic flux than 6E10 and 4G8. Both the current analytical methodology and antibody binding profiles are important for advances in antibody drug development and kinetic biomarker applications for Alzheimer’s disease.

DOI
10.1016/j.ab.2015.03.032
Version
pre-print, post-print
Citation Information
Amanda C. Crisostomo, Loan Dang, Jyothi L. Digambaranath, Andrea C. Klaver, et al.. "Kinetic analysis of IgG antibodies to beta-amyloid oligomers with surface plasmon resonance" Analytical Biochemistry Vol. 481 (2015) p. 43 - 54
Available at: http://works.bepress.com/john_finke/25/