Changes in deflection and strain during a bridge's life cycle can occur due to time dependent strength losses of the bridge's materials. A live load test can be used to determine bench mark values at early-ages and used to compare them to later-age values to determine any changes that may have occurred throughout a bridge's life cycle. Prestressed precast pedestrian bridge constructed with High Strength Concrete (HSC) in Rolla, Missouri, USA was utilized in this study. Both mild steel and Glass Fiber Reinforced Polymer (GFRP) reinforcement were used in the deck system. A static live load test was utilized to compare the deflection in HSC prestressed precast spandrel beams and precast deck panels. In addition, changes in deflection were compared between deck panels reinforced with mild steel to those reinforced with GFRP. Furthermore, the measured values were compared to theoretical values determined from the basic structural analysis for both simply supported and fixed cases.