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Age-dependent Changes in the Articular Cartilage and Subchondral Bone of C57BL/6 Mice after Surgical Destabilization of Medial Meniscus
University of Massachusetts Medical School Faculty Publications
  • Henry Huang, University of Massachusetts Medical School
  • Jordan D. Skelly, University of Massachusetts Medical School
  • David C. Ayers, University of Massachusetts Medical School
  • Jie Song, University of Massachusetts Medical School
UMMS Affiliation
Department of Orthopedics and Physical Rehabilitation; School of Medicine
Date
2-9-2017
Document Type
Article
Abstract

Age is the primary risk factor for osteoarthritis (OA), yet surgical OA mouse models such as destabilization of the medial meniscus (DMM) used for evaluating disease-modifying OA targets are frequently performed on young adult mice only. This study investigates how age affects cartilage and subchondral bone changes in mouse joints following DMM. DMM was performed on male C57BL/6 mice at 4 months (4 M), 12 months (12 M) and 19+ months (19 M+) and on females at 12 M and 18 M+. Two months after surgery, operated and unoperated contralateral knees were harvested and evaluated using cartilage histology scores and muCT quantification of subchondral bone plate thickness and osteophyte formation. The 12 M and 19 M+ male mice developed more cartilage erosions and thicker subchondral bone plates after DMM than 4 M males. The size of osteophytes trended up with age, while the bone volume fraction was significantly higher in the 19 M+ group. Furthermore, 12 M females developed milder OA than males as indicated by less cartilage degradation, less subchondral bone plate sclerosis and smaller osteophytes. Our results reveal distinct age/gender-dependent structural changes in joint cartilage and subchondral bone post-DMM, facilitating more thoughtful selection of murine age/gender when using this surgical technique for translational OA research.

Rights and Permissions
© The Author(s) 2017. Citation: Sci Rep. 2017 Feb 9;7:42294. doi: 10.1038/srep42294. Link to article on publisher's site
Related Resources
Link to Article in PubMed
Keywords
  • Animal disease models,
  • Experimental models of disease,
  • Osteoarthritis
PubMed ID
28181577
Creative Commons License
Creative Commons Attribution 4.0
Citation Information
Henry Huang, Jordan D. Skelly, David C. Ayers and Jie Song. "Age-dependent Changes in the Articular Cartilage and Subchondral Bone of C57BL/6 Mice after Surgical Destabilization of Medial Meniscus" Vol. 7 (2017) ISSN: 2045-2322 (Linking)
Available at: http://works.bepress.com/jie_song/49/