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Article
Cytocompatible poly(ethylene glycol)-co-polycarbonate hydrogels cross-linked by copper-free, strain-promoted click chemistry
Orthopedics and Physical Rehabilitation Publications and Presentations
  • Jianwen Xu, University of Massachusetts Medical School
  • Tera M. Filion, University of Massachusetts Medical School
  • Fioleda Prifti, University of Massachusetts Medical School
  • Jie Song, University of Massachusetts Medical School
UMMS Affiliation
Department of Orthopedics and Physical Rehabilitation
Date
10-1-2011
Document Type
Article
Medical Subject Headings
Alkynes; Animals; Azides; Biocompatible Materials; Bone Marrow Cells; Cell Survival; *Click Chemistry; Cyclization; Heterocyclic Compounds, 3-Ring; Hydrogels; Male; Molecular Structure; Polyethylene Glycols; Rats
Abstract
Strategies to encapsulate cells in cytocompatible three-dimensional hydrogels with tunable mechanical properties and degradability without harmful gelling conditions are highly desired for regenerative medicine applications. Here we reported a method for preparing poly(ethylene glycol)-co-polycarbonate hydrogels through copper-free, strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. Hydrogels with varying mechanical properties were formed by "clicking" azido-functionalized poly(ethylene glycol)-co-polycarbonate macromers with dibenzocyclooctyne-functionalized poly(ethylene glycol) under physiological conditions within minutes. Bone marrow stromal cells encapsulated in these gels exhibited higher cellular viability than those encapsulated in photo-cross-linked poly(ethylene glycol) dimethacrylate. The precise control over the macromer compositions, cytocompatible SPAAC cross-linking, and the degradability of the polycarbonate segments make these hydrogels promising candidates for scaffold and stem cell assisted tissue repair and regeneration.
Rights and Permissions
Citation: 24. Link to article on publisher's site
Related Resources
Link to Article in PubMed
PubMed ID
21954076
Citation Information
Jianwen Xu, Tera M. Filion, Fioleda Prifti and Jie Song. "Cytocompatible poly(ethylene glycol)-co-polycarbonate hydrogels cross-linked by copper-free, strain-promoted click chemistry" Vol. 6 Iss. 10 (2011) ISSN: 1861-471X (Linking)
Available at: http://works.bepress.com/jie_song/24/