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Differential expression of cyclin G2, cyclin dependent kinase inhibitor 2C and peripheral myelin protein 22 genes during adipogenesis..pdf
Animal (2014)
  • Jibin Zhang, The Ohio State University
  • Yeunsu Suh, The Ohio State University
  • Young Min Choi, The Ohio State University
  • Jinsoo Ahn, The Ohio State University
  • Michael E Davis, The Ohio State University
  • Kichoon Lee, The Ohio State University
Abstract
Increase of fat cells (FCs) in adipose tissue is attributed to proliferation of preadipocytes or immature adipocytes in the early stage, as well as adipogenic differentiation in the later stage of adipose development. Although both events are involved in the FC increase, they are contrary to each other, because the former requires cell cycle activity, whereas the latter requires cell cycle withdrawal. Therefore, appropriate regulation of cell cycle inhibition is critical to adipogenesis. In order to explore the important cell cycle inhibitors and study their expression in adipogenesis, we adopted a strategy combining the Gene Expression Omnibus (GEO) database available on the NCBI website and the results of quantitative real-time PCR (qPCR) data in porcine adipose tissue. Three cell cycle inhibitors - cyclinG2 (CCNG2), cyclin-dependentkinaseinhibitor2C (CDKN2C) and peripheralmyelinprotein (PMP22) - were selected for study because they are relatively highly expressed in adipose tissue compared with muscle, heart, lung, liver and kidney in humans and mice based on two GEO DataSets (GDS596 and GDS3142). In the latter analysis, they were found to be more highly expressed in differentiating/ed preadipocytes than in undifferentiated preadipocytes in human and mice as shown respectively by GDS2366 and GDS2743. In addition, GDS2659 also suggested increasing expression of the three cell cycle inhibitors during differentiation of 3T3-L1 cells. Further study with qPCR in Landrace pigs did not confirm the high expression of these genes in adipose tissue compared with other tissues in market-age pigs, but confirmed higher expression of these genes in FCs than in the stromal vascular fraction, as well as increasing expression of these genes during in vitro adipogenic differentiation and in vivo development of adipose tissue. Moreover, the relatively high expression of CCNG2 in adipose tissue of market-age pigs and increasing expression during development of adipose tissue was also confirmed at the protein level by western blot analysis. Based on the analysis of the GEO DataSets and results of qPCR and Western blotting we conclude that all three cell cycle inhibitors may inhibit adipocyte proliferation, but promote adipocyte differentiation and hold a differentiated state by inducing and maintaining cell cycle inhibition. Therefore, their expression in adipose tissue is positively correlated with age and mature FC number. By regulating the expression of these genes, we may be able to control FC number, and, thus, reduce excessive fat tissue in animals and humans.
Keywords
  • cyclin G2 (CCNG2),
  • 22-kDa peripheral myelin protein (PMP22),
  • cyclin-dependent inhibitor 2C (CDKN2C),
  • adipocyte,
  • GEO datasets
Publication Date
Summer May 5, 2014
DOI
10.1017/S1751731114000469.
Citation Information
Jibin Zhang, Yeunsu Suh, Young Min Choi, Jinsoo Ahn, et al.. "Differential expression of cyclin G2, cyclin dependent kinase inhibitor 2C and peripheral myelin protein 22 genes during adipogenesis..pdf" Animal Vol. 8 Iss. 5 (2014) p. 800 - 809 ISSN: 1751-7311
Available at: http://works.bepress.com/jibin-zhang/9/