66th National Poultry Breeder Roundtable Poster_Jibin Zhang.pdfUS Poultry (2017)
Fast-growing modern broilers are susceptible to cardiac arrhythmia and heart failure. To understand the developmental regulation related to these problems, cardiac gene expression in Illinois and Ross broilers was investigated at 6 and 21 days post hatch (dph) using RNA-seq (n=5-6 for each group). Because Illinois is a legacy broiler line with isometric heart growth, and Ross is a modern broiler line with a negative allometric heart growth, comparison of gene expression between the two lines and the two time points provides an avenue to understand genetic regulation underlying their different cardiac development.
Sequence data were mapped to the Galgal5 reference genome and differential expression analysis was performed using edgeR software, with false discovery rate (FDR) < 0.05 and Log2 fold change (Log2FC) > 1. A total of 501 and 388 differentially expressed genes (DEGs) were found between times in Ross and Illinois broilers, respectively, with 138 DEGs shared between the two lines. A total of 170 and 210 DEGs were found between the broiler lines at 6 and 21 dph, respectively, with 28 DEGs shared between the two time points. Among these DEGs, granzyme K always showed the highest, and hemoglobin the lowest, Log2FC in the 21 dph vs. 6 dph contrast. Ribosome Assembly Factor MRTO4 always showed distinctly higher expression in Ross than Illinois broilers.
Ingenuity Pathway Analysis (IPA) predicted development of immune cells from 6 to 21 dph in both broiler lines. A significant decrease in cell proliferation was predicted in Ross but not Illinois broilers from 6 to 21 dph. Cell quantity, apoptosis, and activation of immune cells were all predicted to be higher in Ross than Illinois broilers at 6 dph, whereas only differentiation of connective tissue cells was predicted to be higher in Ross than Illinois broilers at 21 dph. These identified DEGs and pathways related to cardiac development provide potential targets for improving cardiac function in modern broilers through breeding for favorable genetic variants.
- Cardiac development,
- Gene expression,
Publication DateSpring May 4, 2017
LocationSt. Louis, Missouri
Citation InformationJibin Zhang, Carl J. Schmidt and Susan J. Lamont. "66th National Poultry Breeder Roundtable Poster_Jibin Zhang.pdf" US Poultry (2017)
Available at: http://works.bepress.com/jibin-zhang/20/