Identification of CTLA2A, DEFB29, WFDC15B, SERPINA1F and MUP19 as novel tissue-specific secretory factors in mouse..pdfPLoS One (2015)
Secretoryfactors in animals play an important role in communication between different cells, tissues and organs. Especially, the secretoryfactors with specific expression in one tissue may reflect important functions and unique status of that tissue in an organism. In this study, we identified potential tissue-specificsecretoryfactors in the fat, muscle, heart, lung, kidney and liver in the mouse by analyzing microarray data from NCBI's Gene Expression Omnibus (GEO) public repository and searching and predicting their subcellular location in GeneCards and WoLF PSORT, and then confirmed tissue-specific expression of the genes using semi-quantitative PCR reactions. With this approach, we confirmed 11 lung, 7 liver, 2 heart, 1 heart and muscle, 7 kidney and 2 adipose and liver-specific secretoryfactors. Among these genes, 1 lung-specific gene--CTLA2A (cytotoxic T lymphocyte-associated protein 2 alpha), 3 kidney-specific genes--SERPINA1F (serpin peptidase inhibitor, Clade A, member 1F), WFDC15B (WAP four-disulfide core domain 15B) and DEFB29 (defensin beta 29) and 1 liver-specific gene--MUP19 (major urinary protein 19) have not been reported as secretoryfactors. These genes were tagged with hemagglutinin at the 3'end and then transiently transfected to HEK293 cells. Through protein detection in cell lysate and media using Western blotting, we verified secretion of the 5 genes and predicted the potential pathways in which they may participate in the specific tissue through data analysis of GEO profiles. In addition, alternative splicing was detected in transcripts of CTLA2A and SERPINA1F and the corresponding proteins were found not to be secreted in cell culture media. Identification of novelsecretoryfactors through the current study provides a new platform to explore novelsecretoryfactors and a general direction for further study of these genes in the future.
Publication DateSummer May 6, 2015
Citation InformationJibin Zhang, Jinsoo Ahn, Yeunsu Suh, Seongsoo Hwang, et al.. "Identification of CTLA2A, DEFB29, WFDC15B, SERPINA1F and MUP19 as novel tissue-specific secretory factors in mouse..pdf" PLoS One Vol. 10 Iss. 5 (2015) p. 1 - 24 ISSN: 1932-6203
Available at: http://works.bepress.com/jibin-zhang/10/