Article
Persistent Reversal of Enhanced Amphetamine Intake by Transient CaMKII Inhibition
The Journal of Neuroscience
(2013)
Abstract
Amphetamine exposure transiently increases CaMKIIα expression in the nucleus accumbens (NAcc) shell and this persistently increases local GluA1 S831 phosphorylation and enhances behavioral responding to the drug. Here we assessed whether transiently interfering with CaMKII signaling using a dominant-negative CaMKIIα mutant delivered to the NAcc shell with herpes simplex viral (HSV) vectors could reverse these long-lasting biochemical and behavioral effects observed following exposure to amphetamine. As expected, transient expression of CaMKIIα K42M in the NAcc shell produced a corresponding transient increase in CaMKIIα and decrease in pCaMKIIα (T286) protein levels in this site. Remarkably, this transient inhibition of CaMKII activity produced a long-lasting reversal of the increased GluA1 S831 phosphorylation levels in NAcc shell and persistently blocked the enhanced locomotor response to and self-administration of amphetamine normally observed in rats previously exposed to the drug. Together, these results indicate that even transient interference with CaMKII signaling may confer long-lasting benefits in drug sensitized individuals and point to CaMKII and its downstream pathways as attractive therapeutic targets for the treatment of stimulant addiction.
Keywords
- Addiction,
- amphetamine,
- CaMKII,
- GluA1,
- self-administration,
- sensitization,
- substance abuse,
- therapeutics
Disciplines
Publication Date
January 23, 2013
DOI
10.1523/JNEUROSCI.4386-13.2013
Citation Information
Jessica A. Loweth, Dongdong Li, James J. Cortright, Georgia Wilke, et al.. "Persistent Reversal of Enhanced Amphetamine Intake by Transient CaMKII Inhibition" The Journal of Neuroscience Vol. 33 Iss. 4 (2013) p. 1411 - 1416 ISSN: Print: 0270-6474 Online: 1529-2401 Available at: http://works.bepress.com/jessica-loweth/9/
Creative Commons license
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