Interleukin-6 secretion in vitro is up-regulated in ectopic and eutopic endometrial stromal cells from women with endometriosisSurgery Publications and Presentations
UMMS AffiliationDepartment of Surgery
Medical Subject HeadingsAdult; Cells, Cultured; Endometriosis; Endometrium; Estradiol; Female; Humans; Interleukin-1; Interleukin-6; Osmolar Concentration; Stromal Cells
AbstractAn in vitro model developed to compare human endometrial and endometriosis stromal cells was used to examine basal and stimulated expression of interleukin (IL-6). Stromal cells isolated from normal endometrium (NE) exhibited the lowest level of IL-6 secretion (84 pg/10(6) cells-48 h), whereas those cells isolated from endometriosis implants (EI) secreted the highest concentration of this inflammatory cytokine (46,284 pg/10(5) cells-48 h; P < 0.01). Eutopic endometrial stromal cells from women with endometriosis (EE) expressed an intermediate concentration of IL-6 (831 pg/10(6) cells-48 h). Stimulation of the various cultures with IL-1 beta dramatically augmented stromal cell production of IL-6. The mean concentrations of stimulated IL-6 secretion were 16,257, 37,800, and 264,290 pg/10(5) cells-48 h for NE, EE, and EI cells, respectively (P < 0.03). Exposure of the cell cultures to 10 nmol/L estradiol had little direct effect on IL-6 production. The results indicate that endometrial stromal cells isolated from tissues of women with and without endometriosis express IL-6 under basal and cytokine-stimulated conditions. Differential responsiveness among the three cell sources indicates that NE, EE, and EI cells have intrinsic quantitative differences in cytokine regulation.
Rights and PermissionsCitation: J Clin Endocrinol Metab. 1996 Mar;81(3):1118-22. Link to article on publisher's website
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Citation InformationJennifer F. Tseng, Isabella P. Ryan, T. D. Milam, J. T. Murai, et al.. "Interleukin-6 secretion in vitro is up-regulated in ectopic and eutopic endometrial stromal cells from women with endometriosis" Vol. 81 Iss. 3 (1996) ISSN: 0021-972X (Linking)
Available at: http://works.bepress.com/jennifer_tseng/30/