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Tol2 Gene Trap Integrations in the Zebrafish Amyloid Precursor Protein Genes appa and aplp2 Reveal Accumulation of Secreted APP at the Embryonic Veins
Developmental Dynamics
  • Hsin-Kai Liao, Iowa State University
  • Ying Wang, Iowa State University
  • Kristin E. Watt, Iowa State University
  • Qin Wen, Iowa State University
  • Justin Breitback, Iowa State University
  • Chelsy K. Kemmet, Iowa State University
  • Karl J. Clark, Mayo Clinic
  • Stephen C. Ekker, Mayo Clinic
  • Jeffrey J. Essner, Iowa State University
  • Maura McGrail, Iowa State University
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
2-1-2012
DOI
10.1002/dvdy.23725
Abstract
Background—The single spanning transmembrane amyloid precursor protein (APP) and its proteolytic product, amyloid-beta (Aβ) peptide, have been intensely studied due to their role in the pathogenesis of Alzheimer’s disease. However, the biological role of the secreted ectodomain of APP, which is also generated by proteolytic cleavage, is less well understood. Here, we report Tol2 red fluorescent protein (RFP) transposon gene trap integrations in the zebrafish amyloid precursor protein a (appa) and amyloid precursor-like protein 2 (aplp2) genes. The transposon integrations are predicted to disrupt the appa and aplp2 genes to primarily produce secreted ectodomains of the corresponding proteins that are fused to RFP. Results—Our results indicate the Appa-RFP and Aplp2 fusion proteins are likely secreted from the central nervous system and accumulate in the embryonic veins independent of blood flow. Conclusions—The zebrafish appa and aplp2 transposon insertion alleles will be useful for investigating the biological role of the secreted form of APP.
Comments

This is the peer reviewed version of the following article: Liao, H.-K., Wang, Y., Noack Watt, K. E., Wen, Q., Breitbach, J., Kemmet, C. K., Clark, K. J., Ekker, S. C., Essner, J. J. and McGrail, M. (2012), Tol2 gene trap integrations in the zebrafish amyloid precursor protein genes appa and aplp2 reveal accumulation of secreted APP at the embryonic veins. Dev. Dyn., 241: 415–425, which has been published in final form at doi:10.1002/dvdy.23725. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving

Copyright Owner
Wiley Periodicals, Inc.
Language
en
File Format
application/pdf
Citation Information
Hsin-Kai Liao, Ying Wang, Kristin E. Watt, Qin Wen, et al.. "Tol2 Gene Trap Integrations in the Zebrafish Amyloid Precursor Protein Genes appa and aplp2 Reveal Accumulation of Secreted APP at the Embryonic Veins" Developmental Dynamics Vol. 241 Iss. 2 (2012) p. 415 - 425
Available at: http://works.bepress.com/jeffrey_essner/11/