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Article
Characterization of DNA Sequences that Confer Complement Resistance in Leishmania chagasi
Annals of the New York Academy of Sciences
  • Rebecca R. Dahlin-Laborde, Iowa State University
  • Eric Joseph Scolaro, Iowa State University
  • Nathan M. Romine, Iowa State University
  • Amanda Ellen Ramer-Tait, Iowa State University
  • Soi Meng Lei, Iowa State University
  • Jeffrey K. Beetham, Iowa State University
Document Type
Article
Publication Date
12-1-2008
DOI
10.1196/annals.1428.099
Abstract

Serial passage of axenically cultured Leishmania chagasi promastigotes results in a progressive diminution in resistance to complement-mediated lysis (CML), whereas high CML resistance is seen in infectious metacyclic promastigotes from the sandfly vector as well as metacyclic-like promastigotes within low-passage cultures at stationary growth phase. As we previously reported, in a screen seeking to identify novel genes involved in CML resistance: (1) a genomic cosmid library derived from DNA of CML-resistant L. chagasi promastigotes was transfected into high-passage (constitutively CML-sensitive) L. chagasi promastigotes; (2) transformants were screened for acquisition of CML-resistance; (3) multiple cosmid-transfectants exhibited partial CML resistance; and (4) the sequence for one of the cosmids (Cosmid 51) was determined. This report extends the analysis of Cosmid 51, and identifies by deletion analysis a subregion of the cosmid insert that is critical to the CML-resistance phenotype of Cosmid 51 transformants. We also report the sequence determination and initial CML-resistance activity of another cosmid that also confers partial resistance to CML.

Comments

This is a manuscript of an article from Annals of the New York Academy of Sciences 1149 (2008): 347, doi:10.1196/annals.1428.099. Posted with permission.

Copyright Owner
New York Academy of Sciences
Language
en
Date Available
2014-10-10
File Format
application/pdf
Citation Information
Rebecca R. Dahlin-Laborde, Eric Joseph Scolaro, Nathan M. Romine, Amanda Ellen Ramer-Tait, et al.. "Characterization of DNA Sequences that Confer Complement Resistance in Leishmania chagasi" Annals of the New York Academy of Sciences Vol. 1149 (2008) p. 347 - 351
Available at: http://works.bepress.com/jeffrey_beetham/6/