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Development of recombinant viral insecticides by expression of an insect-specific toxin and insect-specific enzyme in nuclear polyhedrosis viruses
Archives of Insect Biochemistry and Physiology (1993)
  • Bruce D. Hammock, University of California, Davis
  • Billy F. McCutchen, University of California, Davis
  • Jeffrey Beetham, University of California, Davis
  • Pabhakara V. Choudary, University of California, Davis
  • Elizabeth Fowler
  • Reiji Ichinose, University of California, Davis
  • Vernon K. Ward, University of California, Davis
  • Joanna M. Vicker, University of California, Davis
  • Bryony C. Bonning, University of California, Davis
  • Lawrence G. Harshman, University of California, Davis
  • David Grant, University of California, Davis
  • Tamon Uematsu, University of California, Davis
  • Susumu Maeda, University of California, Davis
Abstract
As supplements to classical chemical insecticides, two approaches to develop recombinant baculovirus insecticides are described. In one approach an insect-specific toxin is expressed leading to a dramatic reduction in time to death. In the second approach an insect juvenile hormone esterase is expressed which leads to a reduction in feeding. Modifications of the wildtype esterase led to viruses which reduced the time to death as efffectively as did the toxin-expressing virus. In both cases existing recombinant viruses are viewed as leads, and approaches to further improvement in the engineered viruses are suggested. Many of these approaches are based on analogy with the development of classical synthetic insecticides. Using these viruses as examples, the potential utility and limitations of recombinant viruses and other biological insecticides are discussed.
Disciplines
Publication Date
1993
Citation Information
Bruce D. Hammock, Billy F. McCutchen, Jeffrey Beetham, Pabhakara V. Choudary, et al.. "Development of recombinant viral insecticides by expression of an insect-specific toxin and insect-specific enzyme in nuclear polyhedrosis viruses" Archives of Insect Biochemistry and Physiology Vol. 22 Iss. 3–4 (1993)
Available at: http://works.bepress.com/jeffrey_beetham/2/