Article
Analysis of gene expression in wild-type and Notch1 mutant retinal cells by single cell profiling
Developmental Dynamics
(2013)
Abstract
Background: The vertebrate retina comprises sensory neurons, the photoreceptors, as well as many other types of neurons and one type of glial cell. These cells are generated by multipotent and restricted retinal progenitor cells (RPCs), which express Notch1. Loss of Notch1 in RPCs late during retinal development results in the overproduction of rod photoreceptors at the expense of interneurons and glia. Results: To examine the molecular underpinnings of this observation, microarray analysis of single retinal cells from wild-type or Notch1 conditional knockout retinas was performed. In situ hybridization was carried out to validate some of the findings. Conclusions: The majority of Notch1-mutant cells lost expression of known Notch target genes. These cells also had low levels of RPC and cell cycle genes, and robustly up-regulated rod precursor genes. In addition, single wild-type cells, in which cell cycle marker genes were down-regulated, expressed markers of both rod photoreceptors and interneurons.
Keywords
- retina,
- progenitor,
- microarray,
- cell fate,
- Notch
Disciplines
Publication Date
October, 2013
DOI
10.1002/dvdy.24006
Publisher Statement
Copyright 2013 The Authors
Citation Information
Karolina Mizeracka, Jeffrey M. Trimarchi, Michael B. Stadler and Constance L. Cepko. "Analysis of gene expression in wild-type and Notch1 mutant retinal cells by single cell profiling" Developmental Dynamics Vol. 242 Iss. 10 (2013) p. 1147 - 1159 Available at: http://works.bepress.com/jeffrey-trimarchi/2/
Creative Commons license
This work is licensed under a Creative Commons CC_BY-NC-ND International License.