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Effect of Biofield Energy Healing Treatment (The Trivedi Effect®) Based Herbomineral Formulation on Pro-Inflammatory Cytokines Expression in Murine Dendritic and Splenocyte Cells
American Journal of Health Research (2016)
  • Mahendra Kumar Trivedi, Trivedi Global Inc.
  • Alice Branton, Trivedi Global Inc.
  • Dahryn Trivedi, Trivedi Global Inc.
  • Gopal Nayak, Trivedi Global Inc.
  • Cathryn Nykvist, Trivedi Global Inc.
  • Celine Lavelle, Trivedi Global Inc.
  • Daniel Paul Przybylski, Trivedi Global Inc.
  • Dianne Heather Vincent, Trivedi Global Inc.
  • Dorothy Felger, Trivedi Global Inc.
  • Douglas Jay Konersman, Trivedi Global Inc.
  • Elizabeth Ann Feeney, Trivedi Global Inc.
  • Jay Anthony Prague, Trivedi Global Inc.
  • Joanne Lydia Starodub, Trivedi Global Inc.
  • Karan Rasdan, Trivedi Global Inc.
  • Karen Mie Strassman, Trivedi Global Inc.
  • Leonid Soboleff, Trivedi Global Inc.
  • Maire Anne Mayne, Trivedi Global Inc.
  • Mary M. Keesee, Trivedi Global Inc.
  • Padmanabha Narayana Pillai, Trivedi Global Inc.
  • Pamela Clarkson Ansley, Trivedi Global Inc.
  • Ronald David Schmitz, Trivedi Global Inc.
  • Sharyn Marie Sodomora, Trivedi Global Inc.
  • Sambhu Charan Mondal, Trivedi Science Research Laboratory Pvt. Ltd.
  • Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd.
Abstract
The utilization and demand of self-medication with herbomineral-based formulations have increased day-by-day across the globe over the last decade. A new proprietary herbomineral formulation was prepared with the mixture of minerals (zinc, magnesium, and selenium) and the herbal root extract of ashwagandha. The current study was undertaken to evaluate the Biofield Energy Healing (The Trivedi Effect®) on the test herbomineral formulation using murine dendritic cells (DCs) and splenocytes in vitro. The formulation was divided into two parts, one part was control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The effect of the Biofield Energy Treated formulation in murine cells was monitored with an estimation of pro-inflammatory cytokines levels such as tumor necrosis factor (TNF-α), macrophage inflammatory protein-1α (MIP-1α) and interleukin (IL-1β) in cell culture supernatants along with estimations of non-cytotoxic concentrations of the test formulation by MTT assay. The Biofield Treated formulation showed 114.2%, 122.6%, 141.2%, 127.8%, and 114.1% cell viability at concentrations 1.05, 5.2, 10.5, 25.6, and 51.2 µg/mL, respectively in DCs. Similarly, the Biofield Energy Treated and untreated formulations showed more than 100% cell viability in mice splenocytes at 5 µg/mL. The level of TNF-α in DCs was significantly (p≤0.05) inhibited by 19.21% in the Biofield Treated formulation at concentration 5.2 µg/mL as compared to the untreated test formulation. The level of MIP-1α in LPS induced mice splenocyte cells was reduced by 15.35% in the Biofield Energy Treated formulation at 0.0105 µg/mL as compared to the untreated formulation. Similarly, the level of IL-1β in LPS induced mice splenocyte cells was significantly (p≤0.05) reduced by 31.59% in the Biofield Treated formulation at 1.05 µg/mL as compared to the untreated formulation. Altogether, the results suggest that The Trivedi Effect® (Biofield Energy Healing Treatment) showed significant down-regulation of the tested pro-inflammatory cytokines expression and potentiated the immunosuppressive effect of the treated formulation to modulate the immune system. These data also suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.


Keywords
  • Biofield Energy Healing Treatment,
  • Biofield Energy Healers,
  • The Trivedi Effect®,
  • Immunomodulation,
  • Dendritic Cells,
  • Splenocytes,
  • Cytokines
Disciplines
Publication Date
Winter December 8, 2016
DOI
10.11648/j.ajhr.20160406.15
Citation Information
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Sambhu Charan Mondal, Snehasis Jana, Effect of Biofield Energy Healing Treatment (The Trivedi Effect®) Based Herbomineral Formulation on Pro-Inflammatory Cytokines Expression in Murine Dendritic and Splenocyte Cells, American Journal of Health Research. Vol. 4, No. 6, 2016, pp. 179-188. doi: 10.11648/j.ajhr.20160406.15
Creative Commons license
Creative Commons License
This work is licensed under a Creative Commons CC_BY International License.